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KMID : 0665220150280040650
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Korean Journal of Food and Nutrition
2015 Volume.28 No. 4 p.650 ~ p.657
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Suppressive Effect of Acorn (Quercus acutissima Carr.) Extracts in 3T3-L1 Preadipocytes
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Kim Ji-Yeon
Lee Jin
Lee Chang-Won
Kim Ae-Jung
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Abstract
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This study aimed to investigate the suppressive effect of acorn extracts, by evaluating 70% ethanol extract (AE) and hot water extract (AW) using 3T3-L1 preadipocytes. We applied various levels (0, 100, 200, 300 and 500 μg/mL) of AE and AW to 3T3-L1 preadipocytes. The cell viability of the 3T3-L1 preadipocytes was not affected by up 300 μg/mL of extracts, but was suppressed by level 500 μg/mL of both AE and AW by 20% and 9% respectively. The accumulation of lipid droplets in differentiated 3T3-L1 preadipocytes was dose-dependently suppressed by AE and AW. Especially, at high concentrations (300 μg/mL), AE (42%) was more effective than AW (41%). Reactive oxygen species (ROS) was also dose-dependently suppressed by treatment with AE (58%) and AW (52%). With regard to the mRNA related to differentiated 3T3-L1 preadipocytes, PPAR-γ and aP2 were suppressed by treatment with AE (54 and 40%) and AW (38 and 18%). From our results, acorn extract (AE) has more suppressive effects than AW in differentiated 3T3-L1 preadipocytes. We therefore concluded that acorn has suppressive effects against obesity in differentiated 3T3-L1 cells due to antioxidation.
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KEYWORD
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acorn, Quercus acutissima Carr, antiobesity, 3T3-L1 preadipocytes
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